FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000923187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142811977
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC134273029.402083972270757TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG30575512.140962588873061TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2009230.14069058087473993TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGC1964850.1375829983783503TruSeq Adapter, Index 2 (98% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1834900.1284836215102603TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGC1459110.10217000217005608TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAACTCGTATGC1444860.10117218669971917TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)