FastQCFastQC Report
Tue 4 Apr 2023
EGAF00000923229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59242476
Sequences flagged as poor quality0
Sequence length100
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT7642661.2900642437699599TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA7112821.2006284139778358TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)