FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000923299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105195045
Sequences flagged as poor quality0
Sequence length100
%GC23

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC73466126.983800425200635TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2923120.27787620605134017TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1241360.11800555815152701TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACACAGTGATCTCGTATGC1144480.10879599889899758TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)