FastQCFastQC Report
Tue 24 Jan 2023
EGAF00000923332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186363091
Sequences flagged as poor quality0
Sequence length100
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT129065926.92550865664704TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA8380570.4496904378989936TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2287410.1227394323482218TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTAT2114180.11344413685433023TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTAT1884890.10114073499671669TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)