FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000923338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82071332
Sequences flagged as poor quality0
Sequence length100
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3532117843.03716917863597TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA27316693.3284082680661258TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTCAACAATCTCGTAT5594240.6816314373939977TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT4880460.5946607519419814TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTCAACAATCTCGTAT4354170.5305348279226174TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTAT4140890.5045476781100616TruSeq Adapter, Index 13 (97% over 39bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3944790.4806538292810941TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTAACAATCTCGTAT3905340.4758470351133085TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACAGTCAACAATCTCGTAT3588520.43724403059523875TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAGTCAACAATCTCGTAT3484220.42453557351792465TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTCAACAATCTCGTAT3374660.4111862105515724TruSeq Adapter, Index 13 (97% over 40bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2754490.3356214567103651TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATAGTCAACAATCTCGTAT2517770.3067782547991301TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2500140.304630123463818TruSeq Adapter, Index 13 (97% over 40bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA2345140.28574411342562345TruSeq Adapter, Index 13 (97% over 40bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA1847190.22507128311259772TruSeq Adapter, Index 13 (97% over 40bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1688780.20576978085356284TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT1660780.20235811452408253TruSeq Adapter, Index 13 (97% over 40bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1283150.1563457018097379TruSeq Adapter, Index 13 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG982770.11974583280797733TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTAT950250.11578342605673805TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)