FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000923342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143631916
Sequences flagged as poor quality0
Sequence length100
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT110339147.682076732862075TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA6706470.466920597229936TruSeq Adapter, Index 14 (97% over 44bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2655850.18490667492035684TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTAT1695530.11804688311753775TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGGATCTCGTAT1622100.11293450962528412TruSeq Adapter, Index 14 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTAT1500530.10447051336417458TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1482020.10318180257373995TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)