FastQCFastQC Report
Fri 30 Dec 2022
EGAF00000923382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177119147
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1783035810.066872103895125TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA6429480.3630031032161644TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTAT4202550.23727248415440935TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTAT4185650.2363183241843413TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTGAAACGATCTCGTAT3027660.1709391701169383TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT2695940.1522105343020876TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTGAAACGATCTCGTAT2568700.1450266695333622TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGAAACGATCTCGTAT2375210.13410238476363032TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGAAACGATCTCGTAT2195590.12396118867939218TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTGAAACGATCTCGTAT2152510.12152892764326602TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGAAACGATCTCGTAT2141910.12093046044310499TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)