FastQCFastQC Report
Sat 13 May 2023
EGAF00000923388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19525389
Sequences flagged as poor quality0
Sequence length100
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT6282263.2174826324843004TruSeq Adapter, Index 18 (97% over 40bp)
CGGGGACTTATCAGCCAACCTGTTACTGCCGGCCCACCCCCAGGGCCAGC314180.1609084459213591No Hit
CGGGGACTTATCAGCCAACCTGTTATGTGGCACAAACCCGGATCCGCAGC286600.14678324718652214No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATATCGTAT204390.1046790924370316TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)