FastQCFastQC Report
Sun 14 May 2023
EGAF00000923414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923414
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences28294845
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1134480.4009493602103139TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG507330.17930121193454143TruSeq Adapter, Index 5 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)