FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000923529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC179400.897Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCACTGTGCTGAGATGAGCTCCAATAACAACTTTTTAACTTGGAGCA32290.16145No Hit
GTCAGTCTTACGGAAACGGAGCCCACCTGGCATCTATCCTGAGTTTAAAG25200.126No Hit
TGAAGAAGGCAGGGGCCCTTAGAGTCTTGGTTGCCAAACAGATTTGCAGA21260.10629999999999999No Hit
CTGAAGAAGGCAGGGGCCCTTAGAGTCTTGGTTGCCAAACAGATTTGCAG20190.10095No Hit
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG20130.10065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)