FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000923532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT72000.36No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC64130.32065No Hit
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA54920.2746Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA40260.2013No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC39420.1971No Hit
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA33570.16785No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC33010.16505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT31130.15564999999999998TruSeq Adapter, Index 1 (97% over 36bp)
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG28980.1449No Hit
GTTGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG26190.13094999999999998No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA26110.13055No Hit
GTCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG25400.127No Hit
GTCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTGGAAGCCATCT24210.12105No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA23890.11945TruSeq Adapter, Index 1 (97% over 36bp)
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC23800.11900000000000001No Hit
GTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG22920.11460000000000001No Hit
CCCCTCTGAAATAATGAGCAGATTTAGCCAGGCTAGCAGAAAGGAAGAGG22040.11019999999999999No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA20420.1021No Hit
CCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCCCAT20280.10139999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)