FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000923534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT59640.29819999999999997No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC54040.2702No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC34360.17179999999999998No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA34340.1717No Hit
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA31180.1559No Hit
GTTGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG27710.13855No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC26640.1332No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT24830.12415TruSeq Adapter, Index 1 (97% over 36bp)
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG24680.12340000000000001No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA21860.1093No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC20620.1031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)