FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000923536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA144620.7231TruSeq Adapter, Index 1 (97% over 36bp)
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT85750.42874999999999996No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC82920.4146No Hit
GGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG46230.23115000000000002No Hit
GGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTGGAAGCCATCT45980.2299No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC43200.216No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA43030.21515No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT38500.1925TruSeq Adapter, Index 1 (97% over 36bp)
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA35930.17965No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC33920.1696No Hit
GGGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT33800.169No Hit
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG33710.16855No Hit
GTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG33620.1681No Hit
GTTGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG29620.14809999999999998No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA29170.14585No Hit
GGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTA28890.14445No Hit
GGTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCT28310.14155No Hit
GGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTAGAG27910.13955No Hit
GGTAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT27530.13765No Hit
TGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTG24320.12160000000000001No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC23930.11965No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA22130.11065000000000001No Hit
CCCCTCTGAAATAATGAGCAGATTTAGCCAGGCTAGCAGAAAGGAAGAGG21660.1083No Hit
CCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCCCAT21550.10774999999999998No Hit
CTCCTGTTTTGGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTG21470.10735No Hit
GGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGGGG21060.10529999999999999No Hit
GTTCCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCC20560.1028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)