FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000923610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA130190.6509499999999999No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC83520.4176No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT59060.2953TruSeq Adapter, Index 5 (97% over 36bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC56990.28495No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT48730.24364999999999998No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC46510.23254999999999998No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT37820.18910000000000002No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG27060.1353No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC25850.12925No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA25150.12575No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC24210.12105No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT22050.11025No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC21770.10885No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACAAACATCGATCTCGTAT21500.1075Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)