FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT439182.1959TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA137300.6865TruSeq Adapter, Index 5 (97% over 36bp)
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA46080.2304No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA39800.199No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC37350.18675No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT37050.18525No Hit
CCTGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT33960.1698No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC33300.1665No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT30210.15105TruSeq Adapter, Index 5 (97% over 36bp)
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT30130.15065No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA30030.15015No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC26180.13090000000000002No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT25340.12669999999999998No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT24140.1207No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA23360.1168No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA21050.10525000000000001No Hit
GGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAAT20740.1037No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT20370.10185000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)