FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT475412.37705TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA148020.7401TruSeq Adapter, Index 5 (97% over 36bp)
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT55570.27785000000000004No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA46100.2305No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC37970.18985No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA37690.18845No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT37640.1882No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC33630.16815No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA28890.14445No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC27200.136No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT25070.12535000000000002No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT24300.1215No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA23080.1154No Hit
GGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAAT22690.11345000000000001No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA21500.1075No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT20370.10185000000000001No Hit
GCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGA20150.10074999999999999No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT20110.10055000000000001No Hit

[OK]Adapter Content

Adapter graph