FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA106190.53095No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT77880.38939999999999997TruSeq Adapter, Index 25 (97% over 37bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC70690.35345No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT59120.2956No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC41510.20755No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT36840.1842No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT35500.17750000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA30390.15195No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC28620.1431No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT24280.12140000000000001No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC24000.12No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG22420.1121No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG22090.11045No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC21850.10925No Hit
GTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA21320.10660000000000001No Hit
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGA21220.10610000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)