FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000924071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA109550.54775No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT88040.4402TruSeq Adapter, Index 25 (97% over 37bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC69350.34675No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT59080.2954No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT43990.21995Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC41650.20825000000000002No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT37880.1894No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA32100.1605No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC30130.15065No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT26170.13085No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC24080.1204No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG23480.11739999999999999No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG23020.1151No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC22370.11184999999999999No Hit
GTCCCTCCATCGCAATAACCAGAAGTACAGGAATATTAACCTGTTTTCCA22170.11084999999999999No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTGGTCAATCTCGTAT21650.10824999999999999Illumina Single End PCR Primer 1 (96% over 25bp)
GGTGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGA20990.10495000000000002No Hit
GCCTTGGTATTCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGGTTCC20300.1015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)