FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA94340.4717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT65950.32975TruSeq Adapter, Index 25 (97% over 37bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC61840.30920000000000003No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT54500.2725No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC36020.1801No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT33310.16655No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT31670.15835000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA29040.1452No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC25230.12615No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT21940.1097No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC20410.10204999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)