FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000924111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT26650.13324999999999998Illumina Single End PCR Primer 1 (95% over 22bp)
GGGTTCTACAACCAGCATATCTTCATGGCTGTGAAATTCATGGCTGTGGA24010.12004999999999999No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA21460.10729999999999999No Hit
CTCTTCTGTAGCATCAGGGTACTGGATGTCAGGTCTGCGAAACTTCTTAG20640.10319999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)