FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000924129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACAACCAGCATATCTTCATGGCTGTGAAATTCATGGCTGTGGA46180.2309No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT37710.18855Illumina Single End PCR Primer 1 (96% over 25bp)
CTCGTCTGTAGCATCAGGGTACTGGATGTCAGGTCTGCGAAACTTCTTAG33460.1673No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA23070.11535000000000001No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC21090.10545000000000002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)