FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTAT86240.43119999999999997TruSeq Adapter, Index 5 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA56560.2828No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC54380.27190000000000003No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTA28740.1437TruSeq Adapter, Index 5 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)