FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000924297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCC137540.6877No Hit
CCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCA89120.4456No Hit
GCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCA75350.37675000000000003No Hit
GGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGG74490.37245No Hit
TCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCC66470.33235000000000003No Hit
GGCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTC60760.3038No Hit
CACTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAG53530.26765No Hit
GGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGA44310.22155No Hit
CGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGG38620.1931No Hit
CCACCTCAGAAATGCTGTGCTTCTCGACAGGCTCGCACAGTGGCCGGGGC37620.18810000000000002No Hit
GGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCT34740.1737No Hit
GTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCAC34670.17335No Hit
GCGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGG28160.1408No Hit
CCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCAA27400.13699999999999998No Hit
GGCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAAC26270.13135No Hit
GGTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCA25140.1257No Hit
GCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAACA23700.11850000000000001No Hit
GTCCGGTTTAATTTTTGACTCCGTTGTAATTGCTGGATCAGTTCTAGACA21470.10735No Hit
CGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCATC21180.10590000000000001No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC20950.10475000000000001No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA20130.10065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)