FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTAT93690.46845000000000003TruSeq Adapter, Index 5 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA59580.2979No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC58050.29025No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTA32830.16415TruSeq Adapter, Index 5 (97% over 39bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC22130.11065000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)