FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA210511.05255No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC65170.32585000000000003No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC62310.31155No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT61930.30965TruSeq Adapter, Index 7 (97% over 39bp)
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT45880.2294No Hit
CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA43840.2192No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT43800.219Illumina Single End PCR Primer 1 (96% over 25bp)
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT41590.20795000000000002No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC40040.2002Illumina Single End Adapter 1 (96% over 25bp)
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA38920.1946No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG37890.18944999999999998No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG37590.18795No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT35570.17784999999999998No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG33200.166No Hit
CGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCT32520.1626No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC32240.16119999999999998No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG31060.1553No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC30890.15445No Hit
CCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACC30390.15195No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC29750.14875000000000002No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC29300.1465No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT29180.1459No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA27110.13555No Hit
GTCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCT25710.12855No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT25340.12669999999999998No Hit
GACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTC23800.11900000000000001No Hit
AATGATACGGCGACCACCCAGAACTCCAGTCACACAGCAGAATCTCGTAT22880.1144Illumina Single End PCR Primer 1 (95% over 22bp)
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT22720.1136No Hit
CCCCTGGTTCGCTCCATACACCTATGTATTCAGTGTAAGGTAACCATCTT22410.11205No Hit
ACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT21550.10774999999999998No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC20480.10239999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)