FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000924507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG91530.45765No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA69350.34675No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC39640.1982No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA38470.19235000000000002No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA35730.17864999999999998No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA31040.1552No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT30100.1505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT28240.1412TruSeq Adapter, Index 25 (97% over 38bp)
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC26290.13144999999999998No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG21580.10790000000000001No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT21200.106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)