FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000924521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA203901.0194999999999999No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC112340.5617No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC57720.2886No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG45770.22885000000000003No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT42370.21185No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG39100.1955No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA35580.1779No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC33050.16525No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA29790.14895No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT29570.14785No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG29020.1451No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT28820.1441No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA28640.1432No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC28510.14254999999999998No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT28150.14075No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT24520.12260000000000001No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA24160.1208No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG24110.12054999999999999No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC23740.11869999999999999No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC23630.11815No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG23360.1168No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG23310.11655000000000001No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC22680.1134No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT22650.11325No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC22480.1124No Hit
GGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGAT21600.108No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT20720.1036No Hit
GGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCT20520.1026No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA20220.1011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)