FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000924563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT63550.31775000000000003TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA62050.31024999999999997TruSeq Adapter, Index 5 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA54800.27399999999999997No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT53850.26925Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC36360.1818No Hit
CCCAAATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTT30960.1548No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA21220.10610000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)