FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000924573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000924573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT83000.415Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT62330.31165TruSeq Adapter, Index 5 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA47530.23765000000000003No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA46650.23324999999999999TruSeq Adapter, Index 5 (97% over 36bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC41980.20990000000000003No Hit
CCCAAATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTT28800.14400000000000002No Hit
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA21150.10575000000000001Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC20690.10345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)