FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000925681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000925681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACAGAAATCTCGTAT54890.27444999999999997TruSeq Adapter, Index 23 (97% over 37bp)
GCCCTGGTTTCAAATTCATTGGTAATAAATATGCTAACTTTCTGAATCAA48110.24055No Hit
CCTGGTTTCAAATTCATTGGTAATAAATATGCTAACTTTCTGAATCAAAA23100.1155No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC20070.10035000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)