FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000925795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000925795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA123920.6196No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC120150.60075No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTAT92920.4646TruSeq Adapter, Index 9 (97% over 38bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC62070.31035No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT38130.19065Illumina Single End PCR Primer 1 (96% over 25bp)
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC33170.16585No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT30300.1515No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC29480.1474No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG29120.1456No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTA26460.1323TruSeq Adapter, Index 9 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCAGTTCAATCTCGTAT24730.12365Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT23970.11984999999999998No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG23540.11770000000000001No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT21450.10725000000000001No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCATCAAGTATCTCGTAT21400.107Illumina Single End PCR Primer 1 (96% over 25bp)
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG21320.10660000000000001No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT20980.10490000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)