FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000925803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000925803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA120660.6033000000000001No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC112560.5628No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTAT77610.38805TruSeq Adapter, Index 9 (97% over 38bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC53640.2682No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC30410.15205No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC30210.15105No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT29740.1487No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG25960.1298No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT24080.1204Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT21780.1089No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG21660.1083No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTA21540.1077TruSeq Adapter, Index 9 (97% over 38bp)
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT20840.1042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)