FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000925875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000925875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT111410.55705TruSeq Adapter, Index 22 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA41130.20565000000000003No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC33790.16895No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC28000.13999999999999999No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA25180.12589999999999998TruSeq Adapter, Index 22 (97% over 38bp)
TAATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAA21860.1093Illumina PCR Primer Index 4 (96% over 26bp)
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT21520.10759999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)