FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000925877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000925877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT108400.542TruSeq Adapter, Index 22 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA39170.19585000000000002No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC34550.17275000000000001No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA26720.1336TruSeq Adapter, Index 22 (97% over 38bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC25360.1268No Hit
TAATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAA21970.10985Illumina PCR Primer Index 4 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)