FastQCFastQC Report
Wed 8 May 2024
EGAF00000925985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000925985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases200 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGG47140.23570000000000002No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT39570.19784999999999997TruSeq Adapter, Index 13 (97% over 38bp)
GTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTT37090.18545No Hit
GGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCC36840.1842No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC35470.17734999999999998No Hit
CCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTG29480.1474No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT27910.13955No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC26780.1339No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA25830.12915No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT25830.12915No Hit
CTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTTCAG25170.12585000000000002No Hit
GCCGGCCGTCGCACTCATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGT21250.10625000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)