FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000925987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000925987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGG45960.2298No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT40270.20135TruSeq Adapter, Index 13 (97% over 38bp)
GTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTT34880.1744No Hit
GGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCC33660.1683No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC33630.16815No Hit
CCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTG27630.13815No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC25680.1284No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT25390.12695No Hit
CTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTTCAG25260.1263No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA24880.12440000000000001No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23960.1198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)