FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGG47550.23775No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT44170.22085TruSeq Adapter, Index 13 (97% over 38bp)
GGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCC37740.1887No Hit
GTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTT37730.18864999999999998No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC36730.18365No Hit
CCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTG30370.15185No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT28090.14045No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC28070.14035No Hit
CTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTTCAG26320.1316No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA25770.12885No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT25610.12805No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA20530.10265No Hit
GCCGGCCGTCGCACTCATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGT20230.10115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)