FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG119070.5953499999999999No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA108020.5401No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA94830.47415No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA72970.36485No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT71850.35925No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG48190.24095000000000003No Hit
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC46630.23314999999999997No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT42960.21480000000000002No Hit
GGGTGGATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGA35820.1791No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA34980.1749No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC34780.1739No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTAT34560.17279999999999998TruSeq Adapter, Index 5 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT32530.16265Illumina Single End PCR Primer 1 (96% over 25bp)
CTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGGAGGTTG32270.16135No Hit
CCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACAC30550.15275No Hit
CTCCAGCATTGTAGTTTGTAGCTCGTGTGTTGTAACCACTCTCCCATTTG29290.14645No Hit
GGCGTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTG24720.12359999999999999No Hit
CCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACACC23430.11715No Hit
CCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAG22080.1104No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCCAGTTCAATCTCGTAT21450.10725000000000001Illumina Single End PCR Primer 1 (96% over 25bp)
GGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAG21140.1057No Hit
GCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG20630.10315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)