FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG106420.5321No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA99920.49959999999999993No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA86570.43285No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA65800.329No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT65030.32515No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG43950.21974999999999997No Hit
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC41790.20895000000000002No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT39430.19715000000000002No Hit
GGGTGGATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGA33200.166No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC32850.16425No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA32010.16005No Hit
CTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGGAGGTTG30600.153No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTAT30570.15284999999999999TruSeq Adapter, Index 5 (97% over 38bp)
CCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACAC27610.13805No Hit
CTCCAGCATTGTAGTTTGTAGCTCGTGTGTTGTAACCACTCTCCCATTTG27450.13725No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT25480.12739999999999999Illumina Single End PCR Primer 1 (96% over 25bp)
GGCGTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTG23350.11674999999999999No Hit
CCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACACC21490.10744999999999999No Hit
GGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAG20550.10275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)