FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG109940.5497No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA95200.47600000000000003No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA85750.42874999999999996No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT69930.34965No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA60080.3004No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG44720.22360000000000002No Hit
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC42560.21280000000000002No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT39340.19669999999999999No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA32580.1629No Hit
GGGTGGATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGA30900.1545No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTAT30700.1535TruSeq Adapter, Index 5 (97% over 38bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC30400.152No Hit
CCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACAC28660.1433No Hit
CTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGGAGGTTG28030.14015No Hit
CTCCAGCATTGTAGTTTGTAGCTCGTGTGTTGTAACCACTCTCCCATTTG27270.13635No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT25080.12539999999999998Illumina Single End PCR Primer 1 (96% over 25bp)
GGCGTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTG22400.11199999999999999No Hit
CCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACACC20500.10250000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)