FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT78880.3944TruSeq Adapter, Index 5 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA67780.33890000000000003No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC45330.22665No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT41010.20504999999999998Illumina Single End PCR Primer 1 (96% over 25bp)
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT26700.1335No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA22000.11TruSeq Adapter, Index 5 (97% over 37bp)
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT21240.1062No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC20540.10269999999999999No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA20140.10070000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)