FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT188610.9430499999999999No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT84130.42065No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA81170.40584999999999993No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT74370.37185No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA71260.3563No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT59020.29510000000000003No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG58840.2942No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT56830.28414999999999996No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC56350.28175No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT55260.2763No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA51110.25555No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC51020.2551No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT48510.24255000000000002No Hit
CAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTC42320.21159999999999998No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA38240.1912No Hit
GCAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCT35990.17995No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA31760.1588No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA30850.15425No Hit
GCTGCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTG30230.15115No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT28720.1436No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT28220.1411No Hit
CGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTTAC26350.13175No Hit
CTGGCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCG25730.12865000000000001No Hit
GGGGTCTTTTGCTCTTAAAATGAAGTGAAGCTTTGCAAGTCCATTGTCCA24870.12435No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC24860.1243No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC24730.12365No Hit
GCTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGA24640.1232No Hit
CTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCTTTCAGGCAAG24600.123No Hit
GTGGGATTTTTCCAACAGAGGTTTTTCACAGCATTCCTTCAGTTTACTGG24570.12285No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACAC23550.11775No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT23540.11770000000000001TruSeq Adapter, Index 20 (97% over 38bp)
GCCGTGGTCAGCAGCCATTGTTCATTGATCAGCGTGGCACCTGTGGTGAG22000.11No Hit
GGCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAA21900.1095No Hit
GTTCCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTAT21700.1085No Hit
AGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTCA21240.1062No Hit
GCACATCACATCAACCTCTGGTCTCACCAATCGGGGGAGGTTTGGGTTGT20660.1033No Hit
CTGGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGA20520.1026No Hit
CTTCTGCACAGGGCATTCTTTTTGCTTCAGGATGTTTACAACATTTGCTG20510.10254999999999999No Hit
CCCCAGGTGGTGTTGCTGTCCTTCTCCAATTGTGAGTCTGTTGAATGGGA20360.1018No Hit
GTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGC20330.10165No Hit
CCCAGCCAAAATTCTGTTGTGCCAGTAGGAGACAGATGTCCAAATCCTTC20230.10115No Hit
GGGTTGTCATCTTTGTGTTGCAAGAAGCATTCATTTCTCTCAGGTTCTTG20130.10065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)