FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT187730.9386500000000001No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT85630.42815No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA79650.39825No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT73130.36565000000000003No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA72300.36150000000000004No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT60640.3032No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG57760.2888No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT57450.28725No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC56140.2807No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT55080.2754No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC52080.2604No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA50950.25475No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT48280.2414No Hit
CAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTC41420.2071No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA39660.1983No Hit
GCAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCT35200.17600000000000002No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA32090.16045No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA31030.15514999999999998No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT30560.15280000000000002No Hit
GCTGCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTG28260.1413No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT28150.14075No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC25880.12940000000000002No Hit
CGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTTAC25780.1289No Hit
CTGGCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCG25660.1283No Hit
GGGGTCTTTTGCTCTTAAAATGAAGTGAAGCTTTGCAAGTCCATTGTCCA25350.12675No Hit
GTGGGATTTTTCCAACAGAGGTTTTTCACAGCATTCCTTCAGTTTACTGG25010.12505000000000002No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC24580.12290000000000001No Hit
GCTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGA24390.12195No Hit
CTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCTTTCAGGCAAG23760.1188No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACAC23240.11620000000000001No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT22300.11150000000000002TruSeq Adapter, Index 20 (97% over 38bp)
GCCGTGGTCAGCAGCCATTGTTCATTGATCAGCGTGGCACCTGTGGTGAG22170.11084999999999999No Hit
CTTCTGCACAGGGCATTCTTTTTGCTTCAGGATGTTTACAACATTTGCTG21370.10685No Hit
GCACATCACATCAACCTCTGGTCTCACCAATCGGGGGAGGTTTGGGTTGT21300.1065No Hit
GTTCCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTAT21220.10610000000000001No Hit
GGCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAA21220.10610000000000001No Hit
AGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTCA21100.1055No Hit
CTGGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGA20990.10495000000000002No Hit
CCCCAGGTGGTGTTGCTGTCCTTCTCCAATTGTGAGTCTGTTGAATGGGA20360.1018No Hit
GTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGC20250.10124999999999999No Hit
GGGTTGTCATCTTTGTGTTGCAAGAAGCATTCATTTCTCTCAGGTTCTTG20020.1001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)