FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT127100.6355000000000001No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT78400.392No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA73940.36970000000000003No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT69470.34735No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA67110.33555No Hit
CCTGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT65460.3273No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT55550.27775No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC54990.27495No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG54220.2711No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC48720.24359999999999998No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA47210.23605No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT45950.22975No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT38050.19025No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA37070.18535No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT36520.18259999999999998No Hit
GCAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCT32980.16490000000000002No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA30590.15295No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA30110.15055No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT28840.1442No Hit
CAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTC28740.1437No Hit
GCTGCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTG28230.14115No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT26190.13094999999999998No Hit
CGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTTAC26090.13045No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT23960.1198TruSeq Adapter, Index 20 (97% over 38bp)
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC23840.11919999999999999No Hit
GCTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGA22410.11205No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACAC22130.11065000000000001No Hit
CTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCTTTCAGGCAAG21930.10965No Hit
GGTGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT20830.10415No Hit
AGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTCA20760.1038No Hit
GTTCCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTAT20500.10250000000000001No Hit
CTTCTGCACAGGGCATTCTTTTTGCTTCAGGATGTTTACAACATTTGCTG20500.10250000000000001No Hit
GCCGTGGTCAGCAGCCATTGTTCATTGATCAGCGTGGCACCTGTGGTGAG20470.10235000000000001No Hit
GGCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAA20270.10135000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)