FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT115320.5766TruSeq Adapter, Index 10 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA56080.2804No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC45270.22635No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC35670.17834999999999998No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC26090.13045No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT25620.1281No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTA21470.10735TruSeq Adapter, Index 10 (97% over 37bp)
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT20780.10389999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)