FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT69390.34695TruSeq Adapter, Index 25 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA67350.33675No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC64840.3242No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC32300.16149999999999998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTA26300.1315TruSeq Adapter, Index 25 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT23870.11934999999999998Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)