FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTAG57670.28835No Hit
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG53070.26535000000000003No Hit
GCAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAG48040.2402No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA45710.22855000000000003No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC37140.1857No Hit
TGGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTA31850.15925No Hit
CCCCGCTGGAGCCCTGCTGGTTGCTGAGGTAGACGCTGAAGACGGGGCTG30580.1529No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA30420.1521No Hit
GTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGC30230.15115No Hit
CTCACGGATAGACTTAAATTTCTTCAGGGGCACTTTGACCACTGCTGCCT23710.11854999999999999No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA23470.11735000000000001No Hit
GCCGGTGTCAAAAAGGACCAGGAAGTTCTGGGGTGGAGTCCCGATGCTGA23360.1168No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT22700.11349999999999999No Hit
GTAGGCTTAATGTAAAATAACCACATTAGTGAACATTATATCTCTTAGAA20810.10405No Hit
GGGGGCTTCAATTGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA20260.1013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)