FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTAG59460.2973No Hit
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG52650.26325No Hit
GCAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAG52050.26025000000000004No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA45320.22659999999999997No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC42800.214No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA31490.15745No Hit
CCCCGCTGGAGCCCTGCTGGTTGCTGAGGTAGACGCTGAAGACGGGGCTG31330.15664999999999998No Hit
GTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGC30950.15475No Hit
GCCGGTGTCAAAAAGGACCAGGAAGTTCTGGGGTGGAGTCCCGATGCTGA25780.1289No Hit
TGGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCACGGTGCAGTA25090.12545No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT24770.12385No Hit
CTCACGGATAGACTTAAATTTCTTCAGGGGCACTTTGACCACTGCTGCCT24030.12015No Hit
GTAGGCTTAATGTAAAATAACCACATTAGTGAACATTATATCTCTTAGAA22080.1104No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA20220.1011No Hit
GCCCTAGGAGGGTGCAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAG20160.1008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)