FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA148660.7433000000000001TruSeq Adapter, Index 1 (97% over 36bp)
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT91580.4579No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC87550.43775000000000003No Hit
GTGGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG54800.27399999999999997No Hit
GGGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT51760.25880000000000003No Hit
GGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG51120.2556No Hit
GGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTGGAAGCCATCT49990.24995No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC45480.2274No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA45200.22599999999999998No Hit
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA38290.19145No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC37760.1888No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT37210.18605TruSeq Adapter, Index 1 (97% over 36bp)
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG36750.18375No Hit
GGAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTA33240.16620000000000001No Hit
GGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTTAGAG32580.1629No Hit
GGTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCT32190.16095No Hit
GGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGGGG31760.1588No Hit
GGGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTA30380.1519No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA29820.14909999999999998No Hit
GGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGATAGCCACTTAT29420.1471No Hit
GGGCTGTGCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTAC28680.1434No Hit
TGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTG27160.1358No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC26990.13495000000000001No Hit
CCCCTCTGAAATAATGAGCAGATTTAGCCAGGCTAGCAGAAAGGAAGAGG24790.12394999999999999No Hit
GTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGGG22700.11349999999999999No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA22380.1119No Hit
CCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCCCAT22330.11165000000000001No Hit
CTCCTGTTTTGGCCAGGCAGCTCAGCAATAGGAGCAGCCGCATGCTTCTG22250.11125No Hit
CTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCT21580.10790000000000001No Hit
GTTCCCACCCATGGACTTGCCAGACCAGGATCTGTACAGATACATGGCCC20920.1046No Hit
TGTGGAAAGGGATGGCGGGGCAAACATTTAGAGCTAGAAGCCACTACTGG20920.1046No Hit
GCTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCT20400.10200000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)