FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000926969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000926969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA106540.5327000000000001Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GTCCTGTTTGGTTTTTATCCGGGCCAAGCAGAGCTGGCATTCATTCGTAT64590.32295No Hit
GTCGGTACTTGCACAGGAAGTGTTGGCGCTTGTTGCATTCGTTGCTGCTC60250.30125No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTA52870.26435TruSeq Adapter, Index 1 (97% over 36bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC36740.1837No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA36040.1802No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT34140.1707TruSeq Adapter, Index 1 (97% over 36bp)
GTTGGGTGCAGGCAATGGAGAGAGGGCAGAAGGGTGTAGAAGCTGAAGGG32930.16465No Hit
GCAGGAGTTAGCAGAATCTTGATTCTTGCTCTATGGTCGGTACTTGCACA32790.16394999999999998No Hit
GCTGCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTC30040.1502No Hit
GTTGGCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTG25880.12940000000000002No Hit
GTTAGGAGGGAAGAAGGATACTGTGGAAAGGGATGGCGGGGCAAACATTT23460.1173No Hit
GCCTCTTCTGTGGGTCGTGCAGGCCAATCCATATCGGCTGGCTTCTCTGA23280.1164No Hit
GTCGCTTGTTGCATTCGTTGCTGCTCCAAGTTAAAAAGTTGTTATTGGAG22290.11145000000000001No Hit
CTCCGTTTCCGTAAGACTGACACTCGAGCTCGGCATCAGACCAGTTCCTC21550.10774999999999998No Hit
GCCAGGCTAGCAGAAAGGAAGAGGACGGGGCTGTGCAGGAGTTAGCAGAA20160.1008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)